rab39b gene  (Waisman Biomanufacturing)

Journal: Molecular Brain

Article Title: Distribution of Parkinson’s disease associated RAB39B in mouse brain tissue

doi: 10.1186/s13041-020-00584-7

Figure Lengend Snippet: Validation of Rab39b KO mice and monoclonal antibodies. a PCR analysis of Rab39b expression in brain tissue from two independent founder strains confirms complete KO of Rab39b and retained expression of Rab39a and Gapdh . b Evaluation of the specificity of RAB39B antibodies 12,162–1-AP (Proteintec), 1B8 and 1H1 by western blot analysis of human control and RAB39B KO tissue (derived from embryonic stem cell line MEL-1) and mouse wildtype and Rab39b KO tissue

Article Snippet: Quantitative real time PCR (qRT-PCR) analysis was performed with a LightCycler LC480 II (Roche) and taqman probes (Life Technologies) specific for mouse Rab39b (Mm00838519_m1) and Tbp (Mm00446971_m1).

Techniques: Biomarker Discovery, Bioprocessing, Expressing, Western Blot, Control, Derivative Assay

Journal: Molecular Brain

Article Title: Distribution of Parkinson’s disease associated RAB39B in mouse brain tissue

doi: 10.1186/s13041-020-00584-7

Figure Lengend Snippet: Analysis of RAB39B by RT-PCR and western blot. a Quantification of Rab39b expression relative to Tbp in whole mouse brain from 2 weeks to 12 month of age (mean ± SEM, n = 3 per time point). b Representative western blot of steady state RAB39B in whole mouse brain from 2 weeks to 12 month of age. c Quantification of RAB39B steady state levels relative to β-Actin in whole mouse brain from 2 weeks to 12 month of age (mean ± SEM, n = 3 per time point). d Representative western blot of steady state RAB39B in micro-dissected mouse brain ( n = 3 per tissue region). e Quantification of RAB39B steady state levels relative to β-Actin in micro-dissected mouse brain (mean ± SEM, n = 3)

Article Snippet: Quantitative real time PCR (qRT-PCR) analysis was performed with a LightCycler LC480 II (Roche) and taqman probes (Life Technologies) specific for mouse Rab39b (Mm00838519_m1) and Tbp (Mm00446971_m1).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing

Journal: Molecular Brain

Article Title: Distribution of Parkinson’s disease associated RAB39B in mouse brain tissue

doi: 10.1186/s13041-020-00584-7

Figure Lengend Snippet: Distribution of Rab39b by ISH in mouse brain tissue. Representative images of Rab39b localization by ISH in coronal sections of 1 month old mouse brain ( n = 3) at Bregma coordinates ( a ) -1.70 mm, ( b ) -2.30 mm and ( c ) -2.80 mm. Scale bar = 500 μm. ( d - g ) High magnification images of Rab39b localization by ISH at the ( d ) hippocampus, ( e ) cortex, ( f ) caudate putamen and ( g ) SN. Scale bar = 100 μm

Article Snippet: Quantitative real time PCR (qRT-PCR) analysis was performed with a LightCycler LC480 II (Roche) and taqman probes (Life Technologies) specific for mouse Rab39b (Mm00838519_m1) and Tbp (Mm00446971_m1).

Techniques:

Journal: Molecular Brain

Article Title: Distribution of Parkinson’s disease associated RAB39B in mouse brain tissue

doi: 10.1186/s13041-020-00584-7

Figure Lengend Snippet: Distribution of RAB39B by IHC in mouse brain tissue. Representative images of RAB39B localization by IHC in coronal sections of 1 month old mouse brain ( n = 3) at the a cerebral cortex co-stained with MAP2, b , c hippocampus co-stained with MAP2, d SN co-stained with TH, e caudate putamen co-stained with MAP2 and f thalamus co-stained with MAP2. Boxed areas in upper panels (scale bar = 200 μm) are shown at a higher magnification in the corresponding lower panels (scale bar = 50 μm)

Article Snippet: Quantitative real time PCR (qRT-PCR) analysis was performed with a LightCycler LC480 II (Roche) and taqman probes (Life Technologies) specific for mouse Rab39b (Mm00838519_m1) and Tbp (Mm00446971_m1).

Techniques: Staining